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醫(yī)學(xué)論文范文:槐定堿致癇大鼠海馬組織超微結(jié)構(gòu)的觀察
【摘要】 目的 建立槐定堿(sophoridine，SRI)致癇大鼠癲癇模型，電鏡下觀察槐定堿致癇大鼠海馬組織超微結(jié)構(gòu)的變化，探討癲癇發(fā)病機(jī)制。 方法 將30只成年SD大鼠(雌雄不拘)隨機(jī)分為生理鹽水對照組(NS)、槐定堿致癇組(SRI)、苯巴比妥鈉﹢槐定堿組(PBS+SRI)，每組各10只。腹腔注射大劑量槐定堿(47.83mg/kg)后，觀察大鼠行為學(xué)變化以及應(yīng)用電鏡技術(shù)觀察各組大鼠致癇后海馬CA1區(qū)和CA3區(qū)神經(jīng)元組織病理學(xué)和形態(tài)學(xué)的改變。結(jié)果 腹腔注射大劑量槐定堿后，大鼠出現(xiàn)了癇性行為發(fā)作;電鏡下可見槐定堿致癇大鼠海馬CA1區(qū)和CA3區(qū)神經(jīng)元核膜雙層結(jié)構(gòu)破壞,神經(jīng)細(xì)胞核嚴(yán)重固縮,染色質(zhì)顆粒狀聚集,線粒體腫脹,嵴斷裂或消失,毛細(xì)血管內(nèi)皮細(xì)胞核固縮。經(jīng)苯巴比妥鈉干預(yù)后，槐定堿致癇大鼠海馬CA1區(qū)和CA3區(qū)神經(jīng)細(xì)胞的損傷程度減輕。結(jié)論 大劑量槐定堿可導(dǎo)致大鼠在體海馬神經(jīng)細(xì)胞損傷，從而誘發(fā)癲癇。
【關(guān)鍵詞】 槐定堿;癲癇;大鼠;海馬;超微結(jié)構(gòu)
Observation on ultrastructure of morphological deformation on hippocampus in SRI induced epileptic rat
CHEN Xiao-xia, ZHANG Lin-Na, DUAN Hui-yun, et al.(Ningxia Medical University, Yinchuan 750004, China)
[Abstract] Objective To establish a sophoridine (SRI) induced epileptic rats model to observe ultrastructure of morphological deformation on hippocampus in SRI induced epileptic rat.Methods Sprague Dawley rats were randomly divided into saline- control group (n=10), SRI group (n=10) and sodium phenobarbital (PBS)+ SRI group (n=10). Model group was injected with Sophoridien intracerebroventricularly (i.c.v.); saline-control group was injected with physiological saline as model group; sodium Phenobarbital was injected with Phenobarbital after using Sophoridien for 0.5 hour. SRI was intraperitoneally injected in rats and to observe the changes of rat's behavior. Then ultrastructure of morphological deformation of neurons in CA1 and CA3 of hippocampus in saline- control group, SRI group and sodium phenobarbital (PBS) + SRI group rats were observed through electron microscope. Results The kindling model of rats was induced by intraperitoneal injection of SRI. The behavioral seizure was observed, sings of mitochondrial damage, organelle burst and degeneration of synapses were seen at SRI administration; Neurons of PBS+ SRI group were less damages as compared with those of SRI group and most neural structure was undamaged. Conclusion sophoridine can cause hippocampal neurons from being damaged by epileptic seizures in vivo.醫(yī).學(xué)全.在.線網(wǎng)站m.quanxiangyun.cn
[Key words] Sophoridine; Ultrastructure; Hippocampus
