| 公開(公告)號 | CN1130461C |
| 公開(公告)日 | 2003.12.10 |
| 申請(專利)號 | CN00112980.5 |
| 申請日期 | 2000.06.02 |
| 專利名稱 | 一種溶栓酶及其制備方法和應(yīng)用 |
| 主分類號 | C12N15/57 |
| 分類號 | C12N15/57;C12N9/68;A61K38/48 |
| 分案原申請?zhí)? | |
| 優(yōu)先權(quán) | |
| 申請(專利權(quán))人 | 竇德獻(xiàn) |
| 發(fā)明(設(shè)計)人 | 竇德獻(xiàn) |
| 地址 | 100027北京市朝陽區(qū)幸福一村一樓611 |
| 頒證日 | |
| 國際申請 | |
| 進(jìn)入國家日期 | |
| 專利代理機(jī)構(gòu) | 北京三高永信知識產(chǎn)權(quán)代理有限責(zé)任公司 |
| 代理人 | 姚寶琴 |
| 國省代碼 | 北京;11 |
| 主權(quán)項(xiàng) | 一種溶栓酶，其特征在于所述的溶栓酶由395個氨基酸組成，其序列如下：Met Ala Ser Cys Arg Asp Glu Lys Thr Gln 10Met Ile Tyr Gln Gln His Gln Ser Trp Leu 20Arg Pro Val Leu Arg Ser Asn Arg Val Glu 30Tyr Csy Trp Cys Asn Ser Gly Arg Ala Gln 40Cys Ser Glu Gly Asn Ser Asp Cys Tyr Phe 50Gly Asn Gly Ser Ala Tyr Arg Gly Thr His 60Ser Leu Thr Glu Ser Gly Ala Ser Cys Leu 70Pro Trp Asn Ser Met Ile Leu Ile Gly Lys 80Val Tyr Thr Ala Gln Asn Pro Ser Ala Gln 90Ala Leu Gly Leu Gly Lys His Asn Tyr Cys 100Arg Asn Pro Asp Gly Asp Ala Lys Pro Trp 110Cys His Val Leu Lys Asp Arg Arg Leu Thr 120Trp Glu Tyr Cys Asp Val Pro Ser Cys Ser 130Thr Cys Gly Leu Arg Gln Tyr Ser Gln Pro 140Gln Phe Arg Ile Lys Gly Gly Leu Phe Ala 150Asp Ile Ala Ser His Pro Trp Gln Ala Ala 160Ile Phe Ala Lys His Glu Glu Ser Pro Gly 170Glu Arg Phe Leu Cys Gly Gly Ile Leu Ile 180Ser Ser Cys Trp Ile Leu Ser Ala Ala His 190Cys Phe Gln Glu Arg Phe Pro Pro His His 200Leu Thr Val Ile Leu Gly Leu The Tyr Arg 210Val Tyr Pro Gly Glu Glu Glu Gln Lys Phe 220Glu Val Glu Lys Tyr Ile Val His Lys Glu 230Phe Asp Asp Asp Thr Tyr Asp Asn Asp Ile 240Ala Leu Leu Gln Leu Lys Ser Asp Ser Ser 250Arg Cys Ala Gln Glu Ser Ser Val Val Arg 260Thr Val Cys Leu Pro Pro Ala Asp Leu Gln 270Leu Pro Asp Trp Thr Glu Cys Glu Leu Ser 280Gly Tyr Gly Lys His Glu Ala Leu Ser Pro 290Phe Tyr Ser Glu Arg Leu Lys Glu Ala His 300Val Arg Leu Tyr Pro Ser Ser Arg Cys Thr 310Ser Gln His Leu Leu Asn Arg Thr Val Thr 320Asp Asn Met Leu Cys Ala Gly Asp Thr Arg 330Ser Gly Gly Phe Gln Ala Asn Leu His Asp 340Ala Cys Gln Gly Asp Ser Gly Gly Pro Leu 350Val Cys Leu Asn Asp Gly Arg Met Thr Leu 360Val Gly Ile Ile Ser Trp Gly Leu Gly Cys 370Gly Gln Lys Asp Val Pro Gly Val Tyr Thr 380Lys Val Thr Asn Tyr Leu Asp Trp Ile Arg 390Asp Asn Met Arg Pro 395 |
| 摘要 | 本發(fā)明涉及一種新溶栓酶，使用溶栓酶已成為治療心肌梗塞和腦血栓疾病的標(biāo)準(zhǔn)療法。新溶栓酶來源于人類大腦，是經(jīng)重組基因克隆所獲得的新溶栓酶。該分子由大腸桿菌生產(chǎn)。新溶栓酶基因克隆于T7啟動子和lac調(diào)控下表達(dá)。在DnaY的輔助下，新溶栓酶表達(dá)量為細(xì)胞總蛋白的35％以上。新溶栓酶具有組織型纖溶酶原激活劑tPA的優(yōu)點(diǎn)，如血栓特異性的同時，在藥理、藥代和藥效上優(yōu)于tPA。新溶栓酶的半衰期為15至23分鐘，而tPA只有3.5分鐘。 |
| 國際公布 |
